Power supplies are available to meet the power requirements of numerous applications (see below).

The PROTEAN Plus Dodeca Cell allows maximum throughput with the capability to run up to 12 2-D gels at a time The PROTEAN II system provides a choice of glass plates, spacer, and sandwich clamps to cast two gel lengths/ 10 or 20 cm.

Large-format systems - accommodate large gels (up to 20.0 x 18.3 cm for the PROTEAN II system, designed for one-dimensional PAGE) and so offer maximum resolution.

The Criterion System includes the Criterion Cell (for 1–2 gels) and high-throughput Criterion Dodeca cell (for 1–12 gels) both cells are compatible with Criterion precast gels

Midi-format systems - accommodate 13.3 x 8.7 cm gels and offer rapid runs with higher sample capacity and enhanced separation over mini-format gels.

The Mini-PROTEAN System includes the Mini-PROTEAN Tetra cell (with a capacity of up to four gels) and the high-throughput Mini-PROTEAN 3 Dodeca cell (for running up to 12 gels) Use these systems for rapid analysis, method development, or when sample volumes are limited. The short separation distance maximizes the electrical field strength (V/cm) to yield rapid separations with moderate resolution.

Mini-format systems - accommodate small gels (up to 8.6 x 6.7 cm).

Vertical Electrophoresis System Selection Guide Mini-PROTEAN Empty Cassettes and Casting PlatesĬassette Dimensions (for Handcasting Gels) Specifically for the second dimension of 2-d electrophoresis Offers maximum resolution in a single gel and the longest range of separation (with the ability to run up to 12 gels) Multi-cell is available for running up to 6 gels

Large format gel system offers greater resolution over smaller formats and can be used with handcast or precast gelsĬan accommodate up to 4 gels and is available in xi or XL format for running a variety of gel sizes Integrated upper buffer chamber allows leak-free operation Run 1–2 Criterion Precast or handcast gels Wing clamp assembly allows faster setup and leak-free operationįast setup with drop-in gel and cell design (precast or handcast) Run 1–4 precast or handcast gels in the Mini-PROTEAN Tetra cell up to 12 gels in the Mini-PROTEAN Dodeca cell in mini format Vertical Electrophoresis System Selection Guide Mini-PROTEAN System Cell selection is dependent on the requirements for speed, resolution, and throughput (both number of samples and number of gels) as well as the volume of sample available (see Table below). Vertical electrophoresis cells are manufactured in different size formats to accommodate different gels sizes. A USB flash drive can also be used as an alternative storage location or method of transfer for protocols and run data files.Components of a vertical electrophoresis cell. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample-specific IEF conditions. Each protocol can contain up to ten steps in which the voltage, manner of voltage ramping, current, and duration (hr or Vhr) are defined. It is easy to set up runs and to create and edit protocols from the user interface connection to an external computer is not required. The trays also allow sample loading either by inclusion in the rehydration solution (in-gel loading) or with sample cups (sample cup loading). The i12 focusing trays and electrode assemblies accommodate all possible gel configurations (gel-side up or down, with or without electrode wicks). This makes it possible to run different sample types, different pH gradients, and multiple protocols all at the same time. Each channel in the i12 focusing tray is powered by its own power supply, enabling precise control over each IPG strip. The PROTEAN i12 IEF cell can run 1 to 12 IPG strips in 7, 11, 13, 17, 18, and 24 cm focusing trays. The PROTEAN® i12™ IEF system is used for isoelectric focusing (IEF) on immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoretic protein analysis.

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